Journal: The Journal of Experimental Medicine

Article Title: Interleukin 7 Transgenic Mice Develop Chronic Colitis with Decreased Interleukin 7 Protein Accumulation in the Colonic Mucosa

doi:

Figure Lengend Snippet: The phenotype of the infiltrating mucosal lymphocytes at the colitis lesion was analyzed by immunohistochemistry. Sections of colonic tissues from IL-7 transgenic mice were stained with anti-CD4 ( A ) and anti-CD8 ( B ) antibodies. Lymphoid infiltrates in the lamina propria at the colitis lesion were dominated by T cells bearing CD4 + . Both TCR-α/β T cells and γ/δ T cells infiltrated in the lamina propria of colonic mucosa (data not shown).

Article Snippet: mAbs used included anti-CD3, anti-CD4, anti-CD8α, anti-CD8β (Ly-3.2), anti–TCR-α/β, and anti–δ chain of TCR-γ/δ ( PharMingen ).

Techniques: Immunohistochemistry, Transgenic Assay, Staining

Journal: The Journal of Experimental Medicine

Article Title: Interleukin 7 Transgenic Mice Develop Chronic Colitis with Decreased Interleukin 7 Protein Accumulation in the Colonic Mucosa

doi:

Figure Lengend Snippet: The phenotype of the infiltrating mucosal lymphocytes at the colitis lesion was analyzed by immunohistochemistry. Sections of colonic tissues from IL-7 transgenic mice were stained with anti-CD4 ( A ) and anti-CD8 ( B ) antibodies. Lymphoid infiltrates in the lamina propria at the colitis lesion were dominated by T cells bearing CD4 + . Both TCR-α/β T cells and γ/δ T cells infiltrated in the lamina propria of colonic mucosa (data not shown).

Article Snippet: mAbs used included anti-CD3, anti-CD4, anti-CD8α, anti-CD8β (Ly-3.2), anti–TCR-α/β, and anti–δ chain of TCR-γ/δ ( PharMingen ).

Techniques: Immunohistochemistry, Transgenic Assay, Staining

Journal: Nature Communications

Article Title: Neoantigen-specific CD8 T cells with high structural avidity preferentially reside in and eliminate tumors

doi: 10.1038/s41467-023-38946-z

Figure Lengend Snippet: a Computational analysis of TCR features led to the establishment of a predictor of TCR avidity and its application on patients’ TIL-ACT products allowed tracking of predicted low and high-avidity TCRs in post-ACT tumor samples. b Hierarchical clustering of 58 TCR sequences provided based on a biophysical approach . TCRs sharing the closest 4-mer features are next to each other and TCRs recognizing the same pMHC have the same color code. TCR model numbers are presented as labels and further details about TRAV, TRAJ, TRBV, TRBJ, HLA and peptide are found in Supplementary Table . The structural avidity of each cognate TCRs is represented below (mean of n = 3 independent experiments). The black dashed box highlights a region where high-avidity TCRs recognizing multiple pMHC specificities are clustering. c Cumulative analysis for four melanoma patients of the percentage of predicted high-avidity CD8 T cells in blood and tumor samples. Values for individual patients are plotted (gray and black) as well as the cumulative analysis (in red) for which the P value was calculated as described in the method section. The number of clones is indicated below for each patient individually. d Monomeric pMHC-TCR dissociation kinetics of Jurkat cells transfected with neoantigen KIF1B S918F -specific TCR#1 and TCR#2, respectively predicted as high and low-avidity TCRs. e Autologous (Mel8) tumor growth in IL-2 NOG mice adoptively transferred at day 22 with 5 × 10 6 primary T cells transduced with KIF1B S918F -specific TCRs ( n = 1 independent experiment, Mean ± SEM). Log-rank two-sided test was used to determine the exact P value. Source data are provided as a Source Data file.

Article Snippet: KIF1B S918F -specific T cells were obtained by co-transfecting Jurkat cells (Promega) with 500 ng each of TCRα and TCRβ chain RNA together with 300 ng each of CD8α and CD8β RNA, using a Neon electroporation system (Thermo Fisher Scientific) as previously described .

Techniques: Clone Assay, Transfection, Transduction

Journal:

Article Title: CD4 + V?14 natural killer T cells are essential for acceptance of rat islet xenografts in mice

doi:

Figure Lengend Snippet: CD3+CD4+ cells in the liver. CD4/CD3 profiles of hepatic mononuclear cells and splenocytes of B6 mice (a and d). NK1.1 and IL-2Rβ expression on CD3intCD4+ cells (b and e) and CD3brightCD4+ (c and f).

Article Snippet: The following FITC-, biotin-, or phycoerythrin- conjugated mAb’s were purchased from PharMingen Co. (San Diego, California, USA); anti-CD3 (145-2C11), anti–IL-2 receptor β chain (TMβ1), anti-CD4 (GK1.5), anti-CD8α (53-6.7), anti-CD8β (53-5.8), anti-TCRβ (H57-597), and anti-NK1.1(PK136).

Techniques: Expressing

Journal:

Article Title: Characterization of extrathymic CD8?? T cells in the liver and intestine in TAP-1 deficient mice

doi: 10.1046/j.1365-2567.2003.01654.x

Figure Lengend Snippet: Presence of CD8+ T cells at extrathymic sites in TAP-1(−/−) mice. (a) Number of lymphocytes yielded by the spleen, liver, intestine (IEL = intraepithelial lymphocytes) and thymus (n = 4). (b) Phenotypic characterization of lymphocytes. Lymphocytes were obtained from both B6 and TAP-1(−/−) mice at the age of 12 weeks. Two-colour staining for CD4 and CD8α and for TCRαβ and TCRγδ was conducted. Numbers in the figure represent the percentages of fluorescence-positive cells in corresponding areas. The data shown here are representative of three experiments. *P < 0·05.

Article Snippet: The mAbs used here included anti-TCRαβ (H57-597), anti-TCRγδ (GL3), anti-CD3 (145–2C11), anti-IL-2Rβ (IL-2R β-chain; TM-β1), anti-NK1·1 (PK136), anti-CD4 (RM4-5), anti-CD8α (53–6·7) and CD8β (53–5·8) mAbs (PharMingen Co., San Diego, CA).

Techniques: Staining, Fluorescence

Journal:

Article Title: Characterization of extrathymic CD8?? T cells in the liver and intestine in TAP-1 deficient mice

doi: 10.1046/j.1365-2567.2003.01654.x

Figure Lengend Snippet: Further phenotypic characterization of lymphocytes in the livers and spleens of B6 and TAP-1(−/−) mice. (a) Two-colour staining for CD3 and IL-2Rβ and for CD3 and NK1·1. (b) Two-colour staining for CD4 and CD8α and for CD4 and CD8β. (c) Two-colour staining for TCRαβ and TCRγδ. In these experiments, young (12-week-old) and old (40-week-old) B6 and TAP-1(−/−) mice were used to show the expansion of CD8+ extrathymic T cells in the liver. Numbers in the figure represent the percentages of fluorescence-positive cells in corresponding areas. The data shown here are representative of three experiments.

Article Snippet: The mAbs used here included anti-TCRαβ (H57-597), anti-TCRγδ (GL3), anti-CD3 (145–2C11), anti-IL-2Rβ (IL-2R β-chain; TM-β1), anti-NK1·1 (PK136), anti-CD4 (RM4-5), anti-CD8α (53–6·7) and CD8β (53–5·8) mAbs (PharMingen Co., San Diego, CA).

Techniques: Staining, Fluorescence